Microorganisms are virtually ubiquitous on Earth, capable of not only tolerating but adapting to nearly any environmental extreme. subtilis were subjected to various short-term storage experiments, revealing that space-like vacuum and high NaCl concentration negatively affected spore viability. Data from the first 2 years of storage show no significant decrease in spore viability. Desiccated baseline spore samples were also exposed to environmental stresses, including X-rays, 254 nm UV-C, 10% H 2O 2, dry heat (120☌) and wet heat (100☌) to investigate how desiccated spores respond to harsh environmental conditions after long periods of storage. subtilis spores is opened and tested for viability every two years for the first 24 years and then every 25 years until experiment completion.
A set of vials containing a defined concentration of desiccated B. In this study, we report the first data from a 500-year microbial experiment, which started in 2014 and will finish in 2514. subtilis spores can lie dormant while remaining viable, a period that potentially far exceeds the human lifespan is not known although convincing examples of long term spore survival have been recorded. Despite thorough study of spore resistance to external stresses, precisely how long B. subtilis is able to tolerate many environmental extremes by transitioning into a dormant state as spores, allowing survival under otherwise unfavorable conditions. Bacillus subtilis) to challenge the limits of microbial resistance and survival. The ability to form endospores allows certain Gram-positive bacteria (e.g.
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